Two-photon imaging in freely behaving mice
WebMay 11, 2024 · The MINI2P is a two-photon miniscope that enables robust calcium imaging during mouse behavior. Two-photon (2P) microscopy has been the approach of choice … WebNov 16, 2024 · Accanto et al. developed a two-photon fiberscope (2P-FENDO) for the selective and reliable optogenetic photostimulation of neurons and high-speed calcium …
Two-photon imaging in freely behaving mice
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WebJan 1, 2024 · Fast High-resolution Miniature Two-photon Microscopy for Brain Imaging in Freely-behaving ... 展开 . 摘要： We have developed a fast, high-resolution, miniaturized two-photon microscope with a headpiece weighing 2.15 g. It is capable of hour-long recording of neuronal activities down to the level of spines in mice experiencing ... WebAug 14, 2024 · Miniature single-photon imaging systems for brain imaging in freely-behaving animals. A Schematic of the integrated headpiece of a wide-field single-photon …
WebJan 11, 2024 · The long-term imaging of sensory neurons revealed that in the spinal cord, healthy mice demonstrated stereotyped responses. However, in a neuropathic pain model, … WebAstrocytic [Cl −] i changes rapidly in response to awakening. To monitor dynamic changes in astrocytic [Cl −] i in awake freely moving or naturally sleeping mice, we used fibre …
WebHere, using chronically implanted microprisms, we imaged sensory and motor-evoked activity in regions and at speeds inaccessible by other high-resolution imaging techniques. To enable translaminar imaging in freely behaving animals through implanted microprisms, we additionally developed wearable microscopes with custom-compound microlenses. WebUsing a miniature microscope in freely behaving mice and a two-photon microscope in head-fixed, unanesthetized mice, we show that astroglial calcium signals are highest in wake and lowest in sleep ...
WebMay 29, 2024 · With a headpiece weighing 2.15 g and a hollow-core photonic crystal fiber delivering 920-nm femtosecond laser pulses, the FHIRM-TPM is capable of imaging …
WebIn this chapter we focus on various advanced two-photon imaging techniques that were recently developed to improve scanning speed, to enable 3D sampling from large … br24 mediathek sendung verpasstWebTwo-photon fiberscopes can be made sufficiently small and lightweight to be carried by rats and mice and to allow virtually unrestricted movement within a behavioral arena. Typically … br24 digitalradiowecker lenco cr 620WebApr 8, 2024 · In order to investigate the interaction of visual input and heading using 2-photon imaging of somata and axon terminals, we developed a preparation in which mice are head restrained in a moving ... br2450a panasonic batteryWebFiber photometry is a calcium imaging technique that captures 'bulk' or population-level calcium (Ca 2+) activity from specific cell-types within a brain region or functional network in order to study neural circuits Population-level calcium activity can be correlated with behavioral tasks, such as spatial learning, memory recall and goal-directed behaviors. br24 radio heuteWebConducted 2-photon imaging through chronic cranial ... a procedure for performing chronic fluorescence microscopy with cellular-resolution across multiple cortical layers in freely … br24 onlinehttp://cshprotocols.cshlp.org/content/2013/10/pdb.top078147.abstract br24 online radioWebExperienced Scientist and Lecturer in the field of Neuroscience, Biology, Physiology, and Biophysics. Skilled in Scientific Review, Two-photon Microscopy, Laser Safety, … gypset sleeveless tunic shirt