Thioredoxin fusion expression vector
WebApr 1, 2000 · This 1.4 kb fragment was cloned into the high‐expression vector pET32a, under control of the T7 promoter. Expression of this plasmid in Escherichia coli BL21(DE3) resulted in the production of a fusion protein (≈62 kDa) consisting of 112 amino acids of thioredoxin and ≈450 amino acids of fragment C.
Thioredoxin fusion expression vector
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Webmultiple cloning site of the expression vector, pThioHis, are expressed as fusions to a modified version of the E. coli protein thioredoxin (trxA) (Bayer, 1968, Holmgren, 1985). … Webcinereus, and its expression during meiosis Satoshi Namekawa,1 Yosuke Ichijima,2 Fumika Hamada,1 Nobuyuki Kasai,1 Kazuki Iwabata,1 Takayuki Nara,13 Hirobumi Teraoka,2 Fumio Sugawara1 and Kengo Sakaguchi1 Correspondence Kengo Sakaguchi [email protected] 1Department of Applied Biological Science, Faculty of Science …
WebThe improved fusion vector Lf-CATH2-pET32α(-TS) was successfully constructed by firstly deleting the thrombin site and S tag from the pET-32α vector, then inserting the Lf-CATH2 … WebThe pET-32 vector series is designed for high-level expression of recombinant thioredoxin fusion proteins. Previous studies have shown that linkage to thioredoxin increases the yield of biologically active proteins expressed in Escherichia coli. How thioredoxin promotes protein solubility is not known, although a catalytic contribution
WebExpression of human granulocyte macrophage colony stimulating factor (hGMCSF), a cytokine of therapeutic importance, as a thioredoxin (TRX) fusion has been investigated in Escherichia coli BL21 (DE3) codon plus cells. The expression of this protein was low when cloned under the T7 promoter without any fusion tags. WebMar 1, 2009 · To explore a new approach for high expression of the G13 domain, we fused the sequence encoding G13 to thioredoxin (Trx) gene to construct the recombinant expression vector (pThioHisA-G13).
Web2T-T has a TEV-cleavable N-terminal His6-thioredoxin fusion tag. Thioredoxin can improve the solubility of your target protein by promoting correct disulfide bond formation. To …
WebDec 4, 2012 · A thioredoxin fusion protein was expressed and shown to have a different molecular weight compared with native bLf. After purification using Ni-NTA, the yield of recombinant bovine lactoferrin was 15.3 mg/l with a purity of 90.3 %. ... The expression of the bLf was made possible by the Trx fusion in the expression vector pET-32a (pET32bLf … css panama talonario digitalWebThis website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies. csspalm.biocuckoo.orgWebJul 12, 2010 · These NK1 mutants were made with the additional R134G mutation to aid in thioredoxin tag removal and purification . ... was expressed as a 6xHis-thioredoxin fusion protein from the expression vector pET-Duet1 in the Escherichia coli strain Rosetta/gami(DE) (Novagen) to promote disulfide bond formation. The biotinylated … marco lattimoreWebFeb 1, 1993 · Abstract. We have developed a versatile Escherichia coli expression system based on the use of E. coli thioredoxin (trxA) as a gene fusion partner. The broad utility of the system is illustrated by the production of a variety of mammalian cytokines and growth factors as thioredoxin fusion proteins. Although many of these cytokines previously ... css panelWebthioredoxin gene fusion expression vector, pTRXFUS. The DNA sequence is shown for the 3' -end of the thioredoxin gene, the "linker" region encoding an enterokinase (enteropepti … marco latzerWebThe oligonucleotide encoding the peptide was linked to generate different oligomeric oligonucleotide segments containing from one to nine but eight tandem copies which was inserted individually to the E. coli expression vector pET32a. The thioredoxin fusion peptides were successfully expressed and detected with different molecular weight on … marco laubingerWebThis 1.4 kb fragment was cloned into the high-expression vector pET32a, under control of the T7 promoter. Expression of this plasmid in Escherichia coli BL21(DE3) resulted in the production of a fusion protein ( approximately 62 kDa) consisting of 112 amino acids of thioredoxin and approximately 450 amino acids of fragment C. marco lattuada intesa