Cyclin h antibody
WebCyclin H Antibody. Applications: WB, IHC, ICC/IF, IP, ELISA. Reactivity: Human, Mouse, Rat. Publications: 4. Images: 57. Clonality: Polyclonal, Monoclonal, Recombinant … WebAnti-cyclin H Antibody (D-10) is a mouse monoclonal IgG 2b κ cyclin H antibody, cited in 13 publications, provided at 200 µg/ml. raised against full length cyclin H of human origin. Anti-cyclin H Antibody (D-10) is recommended for detection of cyclin H p37 (CAK regulatory subunit) of mouse, rat and human origin by WB, IP, IF, IHC (P) and ...
Cyclin h antibody
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WebAnti-phospho-ERK, anti-ERK and anti-cyclin D1 antibodies were used for analysis of EGF-mediated signaling. The membranes were treated with horseradish peroxidase-conjugated secondary antibody (Jackson ... PANC-1 cells were treated with 10 µg/mL anti-hMUC1 monoclonal antibody for 6 h followed by treatment with 10 ng/mL EGF and incubated for … WebCyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK7 kinase and ring finger protein MAT1. The kinase complex is able to phosphorylate CDK2 and CDC2 kinases, thus functions as ...
WebCyclin E1 (CCNE1) is a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. CCNE1, an essential cyclin activating Cdk2, regulates the G1-S phase transition of the mammalian cell division cycle. Its timing expression plays a direct role in the ... WebRabbit recombinant monoclonal Cyclin H/p34 antibody [EPR3929] conjugated to AP. Validated in Target binding affinity, Antibody labelling. Recombinant AP Anti-Cyclin H …
WebCyclin-H is a protein that in humans is encoded by the CCNH gene. Function. The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic … WebAll lanes : Anti-Cyclin H/p34 antibody [EPR3928] (ab111933) at 1/1000 dilution Lane 1 : Jurkat cell lysate Lane 2 : A431 cell lsyate Lane 3 : K562 cell lysate Lane 4 : HeLa cell …
WebThe active complex of cyclin D/CDK4 targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that …
WebFigure 2. Effect of cyclin D1 on helix 12 mutants of ER. The effect of cyclin D1 on ER mutants was tested in Cos-7 cells (A,B) and in U2-OS cells (C,D) in the presence of ligand.An ERE–TATA–luciferase reporter construct was used in transient transfections together with cyclin D1 and ER 1–535 (A,C) or ER L543/544A mutants (B,D). β … spectrum mobile apple watch 7WebFine granules stained by P35/P25 antibodies were present mainly in the somas of the RGCs of the sham control. These granules were immunoreactive to P35/P25 and appeared to be coarser on days 1 and 3. ... Fukiyama Y, Mimura M, Takai S, Kida T, Oku H. Roscovitine, a Cyclin-Dependent Kinase-5 Inhibitor, Decreases Phosphorylated Tau … spectrum mobile asheboro ncWebView All Images (6) ×. Immunofluorescent analysis of (4% PFA ) fixed MCF-7 cells using 66391-1-Ig (Cyclin A2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). Save time and replace your secondary antibodies with our FlexAble Antibody Labeling Kits. spectrum mobile add phoneWebCyclin E1 Antibody detects endogenous levels of total cyclin E1 protein. Source / Purification. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids near the amino terminus of human cyclin E1. Antibodies are purified by protein A and peptide affinity chromatography. spectrum mobile apple watch ultraWebDec 27, 2016 · Rabbit monoclonal antibodies against total cyclin D1, p-Cyclin D1 (T286), total GSK3β, p-GSK3β (S9), total ERK1 ... and LoVo cells were exposed to obatoclax (0, 25, 50, 100, 200 nM) for 24 h, followed by cyclin D1 immunoblotting. α-Tubulin levels served as the control for equal loading; (B) Overexpression of cyclin D1 attenuates obatoclax ... spectrum mobile assurant phone numberWebof Cyclins and CKIs. RMG-I, RMG-I-H: same as Figure 1.RMG-I-H cells were pretreated with PD98059 (25 μM), LY294002 (50 μM), or anti-Lewis y antibody (10 μg/mL) for 24 h; (B) Quantification of A. Data were expressed as the mean ± SD from three independent experiments. 3. Discussion The progression of the cell cycle is regulated by many factors. spectrum mobile antennas from motorolaWebNov 25, 2024 · cyclin E Antibody (HE12) is a mouse monoclonal IgG 1 κ, cited in 807 publications, provided at 200 µg/ml; raised against recombinant cyclin E of human origin; Anti-cyclin E Antibody (HE12) is recommended for detection of cyclin E (doublet at 50 kDa and single band at 42 kDa) of mouse, rat and human origin by WB, IP, IF and … spectrum mobile black friday deals 2021